Flotation of monocytes from a leukocyte-rich fraction through a simple discontinuous iodixanol gradient provides a simple method for their purification.
Use of an iodixanol gradient improves both the yield and viability of neurons, important factors in the successful establishment of neuron cultures.
One of the focuses of tissue engineering and regenerative medicine research is the efficient in vitro growth of cells from biopsy material on a plastic substratum, as a prelude to its eventual use clinically. A recent paper by Bruce et al [1] reported on the culture of urothelial cells from non-neurogenic and neurogenic human bladder [...]
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