Density Gradient Media – News and Views

Axis-Shield’s density gradient media – Nycodenz and OptiPrep can provide higher resolution of endosomal compartments than the traditional sucrose gradients. The new Axis-Shield Mini-Review No. 3 [2010] provides a summary of the technology as applied to both tissues and cultured cells. In addition to the routine pre-formed gradients, OptiPrep can also offer a self-generated  mode that makes gradient formation simple and highly reproducible.

 A companion Mini-Review – No. 2 [2010] – provides a reference list of the all the known papers reporting the use of both Nycodenz and OptiPrep in endocytosis analysis. The list is divided into tissue and cell type and, when merited by the number of references, also into functional study.

 Detailed methodologies are given in a series of Axis-Shield Application Sheets that are listed in Mini-Review 2 [2010]. Electronic links to these Application Sheets are provided in Mini-Review 2 [2010].

Written by JohnG
Filed under: Membranes