Density Gradient Media – News and Views

Lipid-rich plasma membrane domains have commonly been isolated from tissues and cultured cells as detergent-resistant membranes (DRMs).

Flotation of monocytes from a leukocyte-rich fraction through a simple discontinuous iodixanol gradient provides a simple method for their purification.

Use of an iodixanol gradient improves both the yield and viability of neurons, important factors in the successful establishment of neuron cultures.

There are considerable advantages to the use of OptiPrep for the purification of rAAV, both in the ease of gradient formation and quality of product.

Axis-Shield’s density gradient media – Nycodenz and OptiPrep can provide higher resolution of endosomal compartments than the traditional sucrose gradients. The new Axis-Shield Mini-Review No. 3 [2010] provides a summary of the technology as applied to both tissues and cultured cells. In addition to the routine pre-formed gradients, OptiPrep can also offer a self-generated  mode [...]

One of the focuses of tissue engineering and regenerative medicine research is the efficient in vitro growth of cells from biopsy material on a plastic substratum, as a prelude to its eventual use clinically. A recent paper by Bruce et al [1] reported on the culture of urothelial cells from non-neurogenic and neurogenic human bladder [...]

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